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Battelle Bioremediation Symposium

Learning Lab:
Using subsurface imaging to guide and monitor bioremediation

  • Todd Halihan - Oklahoma State University
  • Samantha Frandsen - Aestus, LLC
  • Stuart W. McDonald - Aestus, LLC
Using Subsurface Imaging to Guide and Monitor Bioremediation Learning Lab Presentation for Battelle Bioremediation 2025

Objective:

This learning lab is intended to educate, generate exposure, and solicit feedback from environmental professionals regarding the use of electrical imaging to characterize and monitor subsurface bioactivity over time (time-lapse imaging), which enables more efficient and effective bioremediation for the site and project stakeholders. Site managers (engineers, geologists, hydrogeologists, others), responsible parties, regulators, and bioremediation professionals will benefit from this Learning Lab which will have hands on examples of equipment used as well as examples of 3D data visualization.

Description:

Successful bioremediation starts with characterizing the spatial distribution of bioactivity in the subsurface. Understanding the extent of bioactive zones and their redox state are also important as this determines available options and related costs for bioremediation.

Traditional site characterization methods using monitoring wells offer only a limited view of critical biostructures with an extremely limited sample size relative to the size of the site as a whole. They also introduce oxygen into the subsurface which potentially alter bioactivity related conditions proximal to the well, and which can result in an incomplete or skewed understanding of actual subsurface conditions. Incorrect conceptual site models (CSMs) can impact the effectiveness and cost of bioremediation efforts.

Laboratory and field studies have demonstrated the effectiveness of electrical imaging in mapping subsurface bioactivity. These modern tools provide a detailed view of bioactivity structure and interactions, both laterally and vertically.

This learning lab will explore how environmental professionals can leverage electrical imaging to characterize and monitor bioactivity over time (static and time-lapse imaging), which enables more efficient and effective bioremediation for the site and project stakeholders.

Poster:
If National Geographic Imaged Subsurface Biomes:
Electrical Pictures of Microbial Structures

  • Todd Halihan - Oklahoma State University
  • Samantha Frandsen - Aestus, LLC
  • Stuart W. McDonald - Aestus, LLC
If National Geographic Imaged Subsurface Biomes: Electrical Pictures of Microbial Structures Poster for Battelle Bioremediation 2025

Background/Objective:

Subsurface microbial ecology studies have historically taken place either in laboratories or discrete wells at scales of less than a meter. In recent years, field collected electrical imaging datasets have successfully visualized microbial biosignatures in the subsurface at much larger scales of one meter to hundreds of meters. Biogeophysical investigations at the laboratory scale and field scale confirm the electrical structures detected are due to bioactivity and assist in understanding community and composition of the biome. Interpretation and understanding of these biostructures is needed to improve the ability to utilize microbial communities for remediation.

Approach/Activities:

Hundreds of NAPL impacted sites have been characterized by collecting electrical datasets ranging from 0.5-meter to 10-meter resolution. These datasets can be collected as both static electrical resistivity imaging (ERI) datasets and temporal datasets (TERI) to assess changes in subsurface bioactivity over time. Targeted drilling and chemical and biochemical samples are subsequently collected to calibrate the electrical imaging data to varying levels of bioactivity and structure in the subsurface. The resulting data sets are then integrated in 2D and 3D to develop robust conceptual site model (CSM) findings which have been used successfully to support monitored natural attenuation remedies at NAPL sites.

Results/Lessons Learned:

This presentation shows subsurface electrical structures (pictures) associated with bioactivity across a wide range of geologic settings and state of weathering at complex environmental sites. The depicted structures generated by bioactivity can be electrically conductive and/or electrically resistive depending on the activity of the setting. A basic model resulting from this body of evidence is that free product NAPL starts as a subsurface resistive zone that gradually develops conductance around it as a bioactive colony grows around the chemically different location. The electrical conductivity of these bioactive zones is higher than how normal geological or fluid signals present. Additionally, well developed bioactive colonies generate biogases during the degradation processes which can result in highly electrically resistive bubbles of trapped gas in the subsurface.

The patterns are dependent on a range of a subsurface parameters and are difficult to predict a priori. Therefore, the use of ultra-high resolution electrical imaging data (biogeophysics) can be key to understanding complex structure of the biome and optimizing resulting bioremediation strategies.

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Madison Culver
Madison Culver, M.Sc.
Staff Geologist / Field Technician
Since joining Aestus in 2024, Madison has performed various project tasks including field data acquisition, data integration and analysis, 2D and 3D visualization, and project report development.

As an undergraduate at Oklahoma Sate University, Madison utilized electrical resistivity imaging to locate a drilling target for a municipal water well in rural Oklahoma. Her Master’s degree, also from Oklahoma State University, focused on the production and laboratory testing of a novel groundwater tracing particle for use in a sole source aquifer.

Madison previously worked at an environmental consulting firm in Dallas, Texas, where she performed soil/groundwater investigations/reporting, PFAS sampling programs, and Environmental Site Assessments.

Madison is based out of Irving, Texas.
Michelle Lahti
Michelle Lahti, PACE
Administrative Director
Since joining Aestus in 2021, Michelle has supported the CEO and other managers in their day-to-day needs. She also manages human resources and all elements of the many conferences Aestus attends. Michelle works hard to ensure that the needs of stakeholders are addressed in all steps of the business process when working with our clients.
Michelle has a bachelor’s degree in Avionics Engineering Technology from Embry-Riddle Aeronautical University. She also has experience as a small business owner, and over 15 years’ experience as an executive assistant in many different industries. She has her Professional Administrative Certification of Excellence (PACE) through the American Society of Administrative Professionals (ASAP).
Michelle is based out of Wellington, Colorado.
Autumn Town
Autumn M. Town, M.Sc.
Staff Geologist / Field Technician
Autumn performs various project tasks for Aestus, including field data acquisition, data integration and analysis, 2D and 3D visualization, and project report development. Since joining Aestus in 2023, she has helped meet project objectives such as mapping stratigraphy, identifying areas of contamination, and characterizing preferential flow pathways at complex sites across the United States.
As an undergraduate at Oklahoma State University, Autumn utilized electrical resistivity imaging (ERI) to evaluate the hydrogeologic effects of PFAS-containing firefighting foams. While completing her Master’s degree, also at Oklahoma State University, she focused primarily on sedimentology and stratigraphy as she studied the effects of depositional and diagenetic processes on porosity development in a tight gas sandstone reservoir.

Autumn is based out of Tulsa, Oklahoma.
Morgan Love
Morgan Love, M.Sc.
Senior Project Geologist
Morgan has been a part of the Aestus team since 2012. She supports all aspects of data integration for Aestus in 2D and 3D space, including modeling of geophysical, geochemical, and geologic data. She also performs geophysical data processing and develops quality control and automation protocols to ensure data integrity.

Prior to Aestus, Morgan used her environmental geology undergraduate degree working for a water resource company, a geosciences software company, and a seismic data acquisition company. She holds a Master's Degree in Computer Information Systems.

Morgan is based out of Fort Collins, Colorado.
Grant Eastman
Grant Eastman
Equipment Manager / Field Technician
Grant maintains all our equipment and vehicles at a firehouse ready status in order for the field team to be ready to mobilize at moment’s notice. He also troubleshoots any technical issues that come up as well as being a member of the field team.

Grant graduated from Colorado State University with a degree in Watershed Science. After graduating he completed 8 years of military service with the Colorado Army National Guard, including two deployments to the middle east. Before joining Aestus, he was a senior technician building custom automation equipment and wire harness mockups for aerospace companies.

Grant is based out of Milliken, Colorado.
Samantha Frandsen
Samantha Frandsen, M.Sc, P.Gp.
Hydrogeophysicist / Project Manager
Since joining Aestus in 2018, Samantha has performed all aspects of the Aestus project lifecycle, including acquisition field work, processing of geophysical data, data integration, 3D visualization, and reporting. As project manager, she oversees a variety of projects completed at complex sites across the United States and strives to help our clients efficiently leverage Aestus’ high-resolution GeoTrax Survey™ data to improve understanding of subsurface conditions.

Prior to her time with Aestus, she earned her Bachelor’s degree (magna cum laude) in Physics from the University of Richmond and taught high school physics as a Teach for America corps member. She then completed her Master’s degree (Distinction) in Exploration Geophysics at the University of Leeds in the UK. For her thesis, she utilized several different geophysical techniques to characterize the subsurface remains of a former gasworks facility for which she was awarded the GETECH award for best overall performance in the Independent Project and the Association for Industrial Archaeology’s Dissertation Award.

When she’s not investigating what’s below the ground, Samantha enjoys exploring all the above-ground fun her Stateline (South Lake Tahoe), NV home has to offer via trail running, mountain biking, and snowboarding.

Samantha is based out of Stateline, Nevada.
Michael McNair
Michael McNair
Project Geologist / Field Team Leader
Michael has worked on a variety of project types using Aestus’ electrical hydrogeology techniques to help Aestus’ clients succeed relative to characterization of contaminated sites, evaluating geohazards, municipal water well exploration, and monitoring subsurface changes over time. Michael currently manages Aestus’ field operations to collect high quality subsurface electrical imagery (GeoTrax Survey™) at rural, urban, and industrial sites with complex logistics. When not in the field, Michael is a key member of Aestus’ reporting team and leads or assists with 2D/3D data integration, QC, data interpretation, and reporting.

Michael previously worked at an environmental consulting firm in Dallas, Texas, where he performed soil/groundwater investigations in the field and subsequently developed reports. Michael earned a Bachelor of Science in Geology from Baylor University.

Michael is based out of Orlando, Florida.
Todd Halihan
Todd Halihan, Ph.D., P.Gp.
CTO/Data Interpretation Lead
Todd’s focus at Aestus centers on subsurface characterization using electrical hydrogeology and sustainable water supply. He is also a professor and the Sun Company Clyde Wheeler Chair in Hydrogeology at Oklahoma State University. He was the National Ground Water Association’s 2018 McEllhiney Lecturer and he is a founding member of the NGWAU program for groundwater outreach and education.

Dr. Halihan is the recipient of the American Institute of Hydrology’s C.V. Theis Award winner. He is also a professional geophysicist (CA), professional driller (OK) and a PADI divemaster.

Todd is based out of Stillwater, Oklahoma.
Stuart McDonald
Stuart W. McDonald, P.E.
CEO/Project Executive/Contracting
Stuart has dedicated over 20 years of his career overseeing the development of Aestus with the goal of empowering clients worldwide to gain more certainty in subsurface issues to make better technical decisions and save time and money on their projects. He has been involved in managing hundreds of projects for Aestus in the USA, Europe, Asia, and South America.

Stuart spent the first decade of his career as an environmental consultant primarily with Canonie Environmental and Harding Lawson Associates and gained experience performing cradle to grave site characterization and remediation work on high profile Superfund and other projects. This experience was the genesis of founding Aestus in 2001 as it became clear that the environmental industry needed more data density and more certainty for optimal decision-making on projects.

Stuart is based out of Loveland, Colorado.